EIF4A3

Available structures

Ortholog search: PDBe RCSB

List of PDB id codes
2HXY, 2HYI, 2J0Q, 2J0S, 2J0U, 2XB2, 3EX7, 4C9B

Identifiers

Aliases

EIF4A3, DDX48, MUK34, NMP265, NUK34, RCPS, eIF4AIII, eukaryotic translation initiation factor 4A3

External IDs

MGI: 1923731 HomoloGene: 5602 GeneCards: EIF4A3

Gene ontology
Molecular function	• nucleotide binding • ATP-dependent RNA helicase activity • selenocysteine insertion sequence binding • helicase activity • poly(A) binding • RNA stem-loop binding • protein binding • RNA binding • nucleic acid binding • ribonucleoprotein complex binding • poly(A) RNA binding • hydrolase activity • ATP binding • mRNA binding
Cellular component	• cytoplasm • cytosol • nuclear speck • catalytic step 2 spliceosome • membrane • exon-exon junction complex • intracellular ribonucleoprotein complex • neuronal cell body • dendrite • spliceosomal complex • nucleus • nucleoplasm
Biological process	• nuclear-transcribed mRNA poly(A) tail shortening • mRNA transport • negative regulation of translation • associative learning • response to organic cyclic compound • regulation of mRNA binding • mRNA processing • positive regulation of translation • negative regulation of gene expression • negative regulation of excitatory postsynaptic potential • cellular response to brain-derived neurotrophic factor stimulus • cellular response to selenite ion • exploration behavior • RNA secondary structure unwinding • selenocysteine metabolic process • RNA splicing • transport • rRNA processing • embryonic cranial skeleton morphogenesis • negative regulation of selenocysteine insertion sequence binding • nuclear-transcribed mRNA catabolic process, nonsense-mediated decay • regulation of translation • negative regulation of selenocysteine incorporation • mRNA splicing, via spliceosome • RNA export from nucleus • termination of RNA polymerase II transcription • mRNA export from nucleus • mRNA 3'-end processing
Sources:Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

Entrez


9775


192170

Ensembl


ENSG00000141543


ENSMUSG00000025580

UniProt


P38919


Q91VC3

RefSeq (mRNA)


NM_014740


NM_138669

RefSeq (protein)


NP_055555.1


NP_619610.1

Location (UCSC)

Chr 17: 80.14 – 80.15 Mb

Chr 11: 119.29 – 119.3 Mb

PubMed search

^[1]

^[2]

Wikidata

View/Edit Human

View/Edit Mouse

Eukaryotic initiation factor 4A-III is a protein that in humans is encoded by the EIF4A3 gene.^[3]^[4]^[5]

Function

This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4A-I and eIF4A-II, two other members of the DEAD box protein family.^[5]

References

↑ "Human PubMed Reference:".
↑ "Mouse PubMed Reference:".
↑ Holzmann K, Gerner C, Poltl A, Schafer R, Obrist P, Ensinger C, Grimm R, Sauermann G (Feb 2000). "A human common nuclear matrix protein homologous to eukaryotic translation initiation factor 4A". Biochem Biophys Res Commun. 267 (1): 339–44. doi:10.1006/bbrc.1999.1973. PMID 10623621.
↑ Chan CC, Dostie J, Diem MD, Feng W, Mann M, Rappsilber J, Dreyfuss G (Jan 2004). "eIF4A3 is a novel component of the exon junction complex". RNA. 10 (2): 200–9. doi:10.1261/rna.5230104. PMC 1370532. PMID 14730019.
1 2 "Entrez Gene: EIF4A3 eukaryotic translation initiation factor 4A, isoform 3".

Nagase T, Miyajima N, Tanaka A, et al. (1995). "Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1". DNA Res. 2 (1): 37–43. doi:10.1093/dnares/2.1.37. PMID 7788527.
Li Q, Imataka H, Morino S, et al. (1999). "Eukaryotic Translation Initiation Factor 4AIII (eIF4AIII) Is Functionally Distinct from eIF4AI and eIF4AII". Mol. Cell. Biol. 19 (11): 7336–46. PMC 84727. PMID 10523622.
Jurica MS, Licklider LJ, Gygi SR, et al. (2002). "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis". RNA. 8 (4): 426–39. doi:10.1017/S1355838202021088. PMC 1370266. PMID 11991638.
Lee S, Baek M, Yang H, et al. (2002). "Identification of genes differentially expressed between gastric cancers and normal gastric mucosa with cDNA microarrays". Cancer Lett. 184 (2): 197–206. doi:10.1016/S0304-3835(02)00197-0. PMID 12127692.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Palacios IM, Gatfield D, St Johnston D, Izaurralde E (2004). "An eIF4AIII-containing complex required for mRNA localization and nonsense-mediated mRNA decay". Nature. 427 (6976): 753–7. doi:10.1038/nature02351. PMID 14973490.
Ferraiuolo MA, Lee CS, Ler LW, et al. (2004). "A nuclear translation-like factor eIF4AIII is recruited to the mRNA during splicing and functions in nonsense-mediated decay". Proc. Natl. Acad. Sci. U.S.A. 101 (12): 4118–23. doi:10.1073/pnas.0400933101. PMC 384704. PMID 15024115.
Shibuya T, Tange T, Sonenberg N, Moore MJ (2004). "eIF4AIII binds spliced mRNA in the exon junction complex and is essential for nonsense-mediated decay". Nat. Struct. Mol. Biol. 11 (4): 346–51. doi:10.1038/nsmb750. PMID 15034551.
Jin J, Smith FD, Stark C, et al. (2004). "Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization". Curr. Biol. 14 (16): 1436–50. doi:10.1016/j.cub.2004.07.051. PMID 15324660.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Andersen JS, Lam YW, Leung AK, et al. (2005). "Nucleolar proteome dynamics". Nature. 433 (7021): 77–83. doi:10.1038/nature03207. PMID 15635413.
Xia Q, Kong XT, Zhang GA, et al. (2005). "Proteomics-based identification of DEAD-box protein 48 as a novel autoantigen, a prospective serum marker for pancreatic cancer". Biochem. Biophys. Res. Commun. 330 (2): 526–32. doi:10.1016/j.bbrc.2005.02.181. PMID 15796914.
Ballut L, Marchadier B, Baguet A, et al. (2005). "The exon junction core complex is locked onto RNA by inhibition of eIF4AIII ATPase activity". Nat. Struct. Mol. Biol. 12 (10): 861–9. doi:10.1038/nsmb990. PMID 16170325.
Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
Gehring NH, Kunz JB, Neu-Yilik G, et al. (2005). "Exon-junction complex components specify distinct routes of nonsense-mediated mRNA decay with differential cofactor requirements". Mol. Cell. 20 (1): 65–75. doi:10.1016/j.molcel.2005.08.012. PMID 16209946.
Andersen CB, Ballut L, Johansen JS, et al. (2006). "Structure of the exon junction core complex with a trapped DEAD-box ATPase bound to RNA". Science. 313 (5795): 1968–72. doi:10.1126/science.1131981. PMID 16931718.
You KT, Li LS, Kim NG, et al. (2007). "Selective Translational Repression of Truncated Proteins from Frameshift Mutation-Derived mRNAs in Tumors". PLoS Biol. 5 (5): e109. doi:10.1371/journal.pbio.0050109. PMC 1854916. PMID 17456004.
Zhang Z, Krainer AR (2007). "Splicing remodels messenger ribonucleoprotein architecture via eIF4A3-dependent and -independent recruitment of exon junction complex components". Proc. Natl. Acad. Sci. U.S.A. 104 (28): 11574–9. doi:10.1073/pnas.0704946104. PMC 1913901. PMID 17606899.

PDB gallery

2hxy: Crystal structure of human apo-eIF4AIII

2hyi: Structure of the human exon junction complex with a trapped DEAD-box helicase bound to RNA

2j0q: THE CRYSTAL STRUCTURE OF THE EXON JUNCTION COMPLEX AT 3.2 A RESOLUTION

2j0s: THE CRYSTAL STRUCTURE OF THE EXON JUNCTION COMPLEX AT 2.2 A RESOLUTION

2j0u: THE CRYSTAL STRUCTURE OF EIF4AIII-BARENTSZ COMPLEX AT 3.0 A RESOLUTION

Protein biosynthesis: translation (prokaryotic, eukaryotic)

Proteins

Initiation factor

Prokaryotic

Archaeal

aIF1
aIF2
aIF5
aIF6

Eukaryotic

eIF1	eIF1 EIF1AX AY 1B

eIF2	EIF2S1 EIF2S2 EIF2S3 EIF2B1 EIF2B2 EIF2B3 EIF2B4 EIF2B5 EIF-2 kinase eIF2A eIF2D

eIF3	EIF3A B C D E F G H I J K L M

eIF4	EIF4A2 A3 B E1 E2 E3 G1 G2 G3 H

eIF5	EIF5 EIF5A A2 5B

eIF6	EIF6

Elongation factor

Prokaryotic	EF-Tu EF-Ts EF-G

Archaeal	aEF-1, aEF-2

Eukaryotic	EEF-1 EEF1A1 EEF1A2 EEF1A3 EEF1B1 EEF1B2 EEF1B3 EEF1B4 EEF1D EEF1E1 EEF1G EEF2

Release factor

Ribosomal Proteins

Other concepts

This article is issued from Wikipedia - version of the 5/29/2016. The text is available under the Creative Commons Attribution/Share Alike but additional terms may apply for the media files.

EIF4A3

Function

References

Further reading