Bleomycin hydrolase

BLMH

Available structures

Ortholog search: PDBe RCSB

List of PDB id codes
1CB5, 2CB5

Identifiers

Aliases

BLMH, BH, BMH, bleomycin hydrolase

External IDs

MGI: 1345186 HomoloGene: 330 GeneCards: BLMH

Gene ontology
Molecular function	• peptidase activity • hydrolase activity • carboxypeptidase activity • cysteine-type endopeptidase activity • protein binding • cysteine-type peptidase activity • aminopeptidase activity
Cellular component	• extracellular exosome • cytosol • cytoplasm • nucleus
Biological process	• protein polyubiquitination • response to drug • proteolysis • response to toxic substance • homocysteine catabolic process
Sources:Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

Entrez


642


104184

Ensembl


ENSG00000108578


ENSMUSG00000020840

UniProt


Q13867


Q8R016

RefSeq (mRNA)


NM_000386


NM_178645

RefSeq (protein)


NP_000377.1


NP_848760.1

Location (UCSC)

Chr 17: 30.25 – 30.29 Mb

Chr 11: 76.92 – 76.99 Mb

PubMed search

^[1]

^[2]

Wikidata

View/Edit Human

View/Edit Mouse

Bleomycin hydrolase is an enzyme that in humans is encoded by the BLMH gene.^[3]^[4]^[5]

Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution. Its biological function is hydrolysis of the reactive electrophile homocysteine thiolactone.^[6] Another of its activities is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily.^[5]

Interactions

BLMH has been shown to interact with RPL29,^[7] RPL11,^[7] UBE2I^[8] and Amyloid precursor protein.^[9]

References

↑ "Human PubMed Reference:".
↑ "Mouse PubMed Reference:".
↑ Ferrando AA, Pendas AM, Llano E, Velasco G, Lidereau R, Lopez-Otin C (January 1998). "Gene characterization, promoter analysis, and chromosomal localization of human bleomycin hydrolase". J Biol Chem. 272 (52): 33298–304. doi:10.1074/jbc.272.52.33298. PMID 9407121.
↑ Montoya SE, Ferrell RE, Lazo JS (October 1997). "Genomic structure and genetic mapping of the human neutral cysteine protease bleomycin hydrolase". Cancer Res. 57 (19): 4191–5. PMID 9331073.
1 2 "Entrez Gene: BLMH bleomycin hydrolase".
↑ Jarosław Zimny; Marta Sikora; Andrzej Guranowski & Hieronim Jakubowski (2006). "Protective Mechanisms against Homocysteine Toxicity". The Journal of Biological Chemistry. 281: 22485–22492. doi:10.1074/jbc.M603656200.
1 2 Koldamova, R P; Lefterov I M; DiSabella M T; Almonte C; Watkins S C; Lazo J S (June 1999). "Human bleomycin hydrolase binds ribosomal proteins". Biochemistry. UNITED STATES. 38 (22): 7111–7. doi:10.1021/bi990135l. ISSN 0006-2960. PMID 10353821.
↑ Koldamova, R P; Lefterov I M; DiSabella M T; Lazo J S (December 1998). "An evolutionarily conserved cysteine protease, human bleomycin hydrolase, binds to the human homologue of ubiquitin-conjugating enzyme 9". Mol. Pharmacol. UNITED STATES. 54 (6): 954–61. ISSN 0026-895X. PMID 9855622.
↑ Lefterov, I M; Koldamova R P; Lazo J S (September 2000). "Human bleomycin hydrolase regulates the secretion of amyloid precursor protein". FASEB J. UNITED STATES. 14 (12): 1837–47. doi:10.1096/fj.99-0938com. ISSN 0892-6638. PMID 10973933.

Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene. 138 (1-2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Ferrando AA, Velasco G, Campo E, Lopez-Otin C (1996). "Cloning and expression analysis of human bleomycin hydrolase, a cysteine proteinase involved in chemotherapy resistance.". Cancer Res. 56 (8): 1746–50. PMID 8620487.
Brömme D, Rossi AB, Smeekens SP, et al. (1996). "Human bleomycin hydrolase: molecular cloning, sequencing, functional expression, and enzymatic characterization.". Biochemistry. 35 (21): 6706–14. doi:10.1021/bi960092y. PMID 8639621.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library.". Gene. 200 (1-2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Koldamova RP, Lefterov IM, Gadjeva VG, Lazo JS (1998). "Essential binding and functional domains of human bleomycin hydrolase.". Biochemistry. 37 (8): 2282–90. doi:10.1021/bi9722204. PMID 9485374.
Montoya SE, Aston CE, DeKosky ST, et al. (1998). "Bleomycin hydrolase is associated with risk of sporadic Alzheimer's disease.". Nat. Genet. 18 (3): 211–2. doi:10.1038/ng0398-211. PMID 9500538.
Zheng W, Johnston SA, Joshua-Tor L (1998). "The unusual active site of Gal6/bleomycin hydrolase can act as a carboxypeptidase, aminopeptidase, and peptide ligase.". Cell. 93 (1): 103–9. doi:10.1016/S0092-8674(00)81150-2. PMID 9546396.
Koldamova RP, Lefterov IM, DiSabella MT, Lazo JS (1999). "An evolutionarily conserved cysteine protease, human bleomycin hydrolase, binds to the human homologue of ubiquitin-conjugating enzyme 9.". Mol. Pharmacol. 54 (6): 954–61. PMID 9855622.
Takeda A, Nonaka M, Ishikawa A, Higuchi D (1999). "Immunohistochemical localization of the neutral cysteine protease bleomycin hydrolase in human skin.". Arch. Dermatol. Res. 291 (4): 238–40. doi:10.1007/s004030050400. PMID 10335922.
Koldamova RP, Lefterov IM, DiSabella MT, et al. (1999). "Human bleomycin hydrolase binds ribosomal proteins.". Biochemistry. 38 (22): 7111–7. doi:10.1021/bi990135l. PMID 10353821.
O'Farrell PA, Gonzalez F, Zheng W, et al. (1999). "Crystal structure of human bleomycin hydrolase, a self-compartmentalizing cysteine protease.". Structure. 7 (6): 619–27. doi:10.1016/S0969-2126(99)80083-5. PMID 10404591.
Malherbe P, Faull RL, Richards JG (2000). "Regional and cellular distribution of bleomycin hydrolase mRNA in human brain: comparison between Alzheimer's diseased and control brains.". Neurosci. Lett. 281 (1): 37–40. doi:10.1016/S0304-3940(00)00802-8. PMID 10686410.
Lefterov IM, Koldamova RP, Lazo JS (2000). "Human bleomycin hydrolase regulates the secretion of amyloid precursor protein.". FASEB J. 14 (12): 1837–47. doi:10.1096/fj.99-0938com. PMID 10973933.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Nivet-Antoine V, Coulhon MP, Le Denmat C, et al. (2003). "[Apolipoprotein E and bleomycin hydrolase. Polymorphisms: association with neurodegenerative diseases]". Ann. Biol. Clin. (Paris). 61 (1): 61–7. PMID 12604387.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network.". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.

PDB gallery

1cb5: HUMAN BLEOMYCIN HYDROLASE.

2cb5: HUMAN BLEOMYCIN HYDROLASE, C73S/DELE455 MUTANT

Proteases: cysteine proteases (EC 3.4.22)

Caspase	Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase 13 Caspase 14

Fruit-derived	Papain Ficain Bromelain Actinidain

Calpain	CAPN1 CAPN2 CAPN3 CAPN5 CAPN6 CAPN7 CAPN8 CAPN9 CAPN10 CAPN11 CAPN12 CAPN13 CAPN14 CAPNS1 CAPNS2

Cathepsin	B C F H K L1/L2 O S W Z

Other	Clostripain Cancer procoagulant Separase Autophagin Cruzipain

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

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Bleomycin hydrolase

Interactions

References

Further reading