Rhamnogalacturonan rhamnohydrolase

Rhamnogalacturonan rhamnohydrolase
Identifiers
EC number 3.2.1.174
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

Rhamnogalacturonan rhamnohydrolase (EC 3.2.1.174, RG-rhamnohydrolase, RG alpha-L-rhamnopyranohydrolase) is an enzyme with systematic name rhamnogalacturonan oligosaccharide alpha-L-Rha-(1->4)-alpha-D-GalA rhamnohydrolase.[1][2] This enzyme catalyses the following chemical reaction

Exohydrolysis of the alpha-L-Rha-(1->4)-alpha-D-GalA bond in rhamnogalacturonan oligosaccharides with initial inversion of configuration releasing beta-L-rhamnose from the non-reducing end of rhamnogalacturonan oligosaccharides.

The enzyme is part of the degradation system for rhamnogalacturonan I in Aspergillus aculeatus.

References

  1. Pitson, S.M.; Mutter, M.; van den Broek, L.A.; Voragen, A.G.; Beldman, G. (1998). "Stereochemical course of hydrolysis catalysed by α-L-rhamnosyl and α-D-galacturonosyl hydrolases from Aspergillus aculeatus". Biochem. Biophys. Res. Commun. 242: 552–559. doi:10.1006/bbrc.1997.8009. PMID 9464254.
  2. Mutter, M.; Beldman, G.; Schols, H.A.; Voragen, A.G. (1994). "Rhamnogalacturonan α-L-rhamnopyranohydrolase. A novel enzyme specific for the terminal nonreducing rhamnosyl unit in rhamnogalacturonan regions of pectin". Plant Physiol. 106: 241–250. doi:10.1104/pp.106.1.241. PMID 7972516.
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