Pitrilysin
| Pitrilysin | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 3.4.24.55 | ||||||||
| CAS number | 81611-78-1 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
Pitrilysin (EC 3.4.24.55, Escherichia coli protease III, protease Pi, proteinase Pi, PTR, Escherichia coli metalloproteinase Pi) is an enzyme.[1][2][3][4][5] This enzyme catalyses the following chemical reaction
- Preferential cleavage of -Tyr16- Leu- and -Phe25- Tyr-bonds of oxidized insulin B chain. Also acts on other substrates of less than 7 kDa such as glucagon
This enzyme is present in bacteria Escherichia coli.
References
- ↑ Finch, P.W.; Wilson, R.E.; Brown, K.; Hickson, I.D.; Emmerson, P.T. (1986). "Complete nucleotide sequence of the Escherichia coli ptr gene encoding protease III". Nucleic Acids Res. 14: 7695–7703. doi:10.1093/nar/14.19.7695. PMID 3534791.
- ↑ Affholter, J.A.; Fried, V.A.; Roth, R.A. (1988). "Human insulin-degrading enzyme shares structural and functional homologies with E. coli protease III". Science. 242: 1415–1418. doi:10.1126/science.3059494. PMID 3059494.
- ↑ Becker, A.B.; Roth, R.A. (1992). "An unusual active site identified in a family of zinc metalloendopeptidases". Proc. Natl. Acad. Sci. USA. 89 (9): 3835–3839. doi:10.1073/pnas.89.9.3835. PMID 1570301.
- ↑ Ding, L.; Becker, A.B.; Suzuki, A.; Roth, R.A. (1992). "Comparison of the enzymatic and biochemical properties of human insulin-degrading enzyme and Escherichia coli protease III". J. Biol. Chem. 267 (4): 2414–2420. PMID 1733942.
- ↑ Anastasi, A.; Knight, C.G.; Barrett, A.J. (1993). "Characterization of the bacterial metalloendopeptidase pitrilysin by use of a continuous fluorescence assay". Biochem. J. 290: 601–607. PMID 7680857.
External links
- Pitrilysin at the US National Library of Medicine Medical Subject Headings (MeSH)
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