Mycobacterium massiliense

Mycobacterium massiliense is a rapidly growing Mycobacteria species sharing an identical 16S rRNA sequence with Mycobacterium abscessus. Etymology: massiliense, pertaining to Massilia, the Latin name of Marseille, France where the organism was isolated.

Description

Strictly aerobic, nonmotile, non-spore forming, acid-fast, Gram-positive rods.

Colony characteristics

• The colonies are nonphotochromogenic and intermediate between smooth and rough, although it is possible to identify isolates with rough phenotype;

Physiology

• Growth occurs between 2 and 4 days on 5% sheep blood agar, Middlebrook 7H10 agar, and LJ slants at between at 24 and 37 °C; no growth is observed at 42 and 45 °C.

Differential Characteristics

• Differentiated from Mycobacterium abscessus by hsp65 sequence (5bp difference)

Type strain

• The type strain was isolated from the sputum of a patient with hemoptoic pneumonia.

Strain CCUG 48898 = CIP 108297.

References

• Adékambi; et al. (2004). "Amoebal coculture of "Mycobacterium massiliense" sp. nov. from the sputum of a patient with hemoptoic pneumonia". J. Clin. Microbiol. 42: 5493–5501. doi:10.1128/JCM.42.12.5493-5501.2004. PMC 535245. PMID 15583272. 
• Viana-Niero, C; Lima, KV; Lopes, ML; Rabello, MC; Marsola, LR; Brilhante, VC; Durham, AM; Leão, SC (Mar 2008). "Molecular characterization of Mycobacterium massiliense and Mycobacterium bolletii in isolates collected from outbreaks of infections after laparoscopic surgeries and cosmetic procedures". J Clin Microbiol. 46 (3): 850–5. doi:10.1128/JCM.02052-07. PMC 2268380. PMID 18174307.