Kappa-carrageenase

Kappa-carrageenase
Identifiers
EC number 3.2.1.83
CAS number 37288-59-8
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

Kappa-carrageenase (EC 3.2.1.83, kappa-carrageenan 4-beta-D-glycanohydrolase) is an enzyme with systematic name kappa-carrageenan 4-beta-D-glycanohydrolase (configuration-retaining).[1][2][3][4][5] This enzyme catalyses the following chemical reaction

Endohydrolysis of (1->4)-beta-D-linkages between D-galactose 4-sulfate and 3,6-anhydro-D-galactose in kappa-carrageenans

The main products of hydrolysis are neocarrabiose-sulfate and neocarratetraose-sulfate.

References

  1. Weigl, J.; Yashe, W. (1966). "The enzymic hydrolysis of carrageenan by Pseudomonas carrageenovora: purification of a κ-carrageenase". Can. J. Microbiol. 12: 939–947. doi:10.1139/m66-127. PMID 5972647.
  2. Potin, P.; Sanseau, A.; Le Gall, Y.; Rochas, C.; Kloareg, B. (1991). "Purification and characterization of a new κ-carrageenase from a marine Cytophaga-like bacterium". Eur. J. Biochem. 201: 241–247. doi:10.1111/j.1432-1033.1991.tb16280.x. PMID 1915370.
  3. Potin, P.; Richard, C.; Barbeyron, T.; Henrissat, B.; Gey, C.; Petillot, Y.; Forest, E.; Dideberg, O.; Rochas, C.; Kloareg, B. (1995). "Processing and hydrolytic mechanism of the cgkA-encoded κ-carrageenase of Alteromonas carrageenovora". Eur. J. Biochem. 228: 971–975. doi:10.1111/j.1432-1033.1995.tb20348.x. PMID 7737202.
  4. Michel, G.; Barbeyron, T.; Flament, D.; Vernet, T.; Kloareg, B.; Dideberg, O. (1999). "Expression, purification, crystallization and preliminary x-ray analysis of the κ-carrageenase from Pseudoalteromonas carrageenovora". Acta Crystallogr. D. 55: 918–920. doi:10.1107/s0907444998018526. PMID 10089334.
  5. Michel, G.; Chantalat, L.; Duee, E.; Barbeyron, T.; Henrissat, B.; Kloareg, B.; Dideberg, O. (2001). "The κ-carrageenase of P. carrageenovora features a tunnel-shaped active site: a novel insight in the evolution of Clan-B glycoside hydrolases". Structure. 9: 513–525. doi:10.1016/s0969-2126(01)00612-8. PMID 11435116.
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