Seminaphtharhodafluor

Seminaphtharhodafluor

Names
Other names Seminaphtharhodafluorescein; Seminaphthorhodafluor
Identifiers
CAS Number	153967-04-5 Y
3D model (Jmol)	Interactive image
SMILES O=C(O1)C2=C(C=CC=C2)C31C4=CC=C(N)C=C4OC5=C3C=CC6=C5C=CC(O)=C6
Properties
Chemical formula	C24H15NO4
Molar mass	381.39 g·mol−1
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
Infobox references

Seminaphtharhodafluor or SNARF is a fluorescent dye that changes color with pH. It can be used to construct optical biosensors that use enzymes that change pH.

The absorption peak of the derivative carboxy-SNARF at pH 6.0 is at wavelength (515 and) 550 nm, while that at pH 9.0 is at 575 nm.

The emission peak of carboxy-SNARF at pH 6.0 is at wavelength 585 nm, while that at pH 9.0 is at 640 nm.^[1]

SNARF-1 can serve as a substrate for the MRP1 (multidrug resistance-associated protein-1) drug transporter, to measure the activity of the MRP1 transporter. For this purpose, an acetomethoxyester group is added to SNARF-1. Cellular esterases cleave off SNARF-1, and its transport out of the cells can be measured by following the loss of fluorescence from the cells.^[2]

References