Microbial mat

The cyanobacterial-algal mat, salty lake on the White Sea seaside

A microbial mat is a multi-layered sheet of microorganisms, mainly bacteria and archaea. Microbial mats grow at interfaces between different types of material, mostly on submerged or moist surfaces, but a few survive in deserts.[1] They colonize environments ranging in temperature from –40 °C to 120 °C. A few are found as endosymbionts of animals.

Although only a few centimetres thick at most, microbial mats create a wide range of internal chemical environments, and hence generally consist of layers of microorganisms that can feed on or at least tolerate the dominant chemicals at their level and which are usually of closely related species. In moist conditions mats are usually held together by slimy substances secreted by the micro-organisms, and in many cases some of the micro-organisms form tangled webs of filaments which make the mat tougher. The best known physical forms are flat mats and stubby pillars called stromatolites, but there are also spherical forms.

Microbial mats are the earliest form of life on Earth for which there is good fossil evidence, from 3,500 million years ago, and have been the most important members and maintainers of the planet's ecosystems. Originally they depended on hydrothermal vents for energy and chemical "food", but the development of photosynthesis gradually liberated them from the "hydrothermal ghetto" by providing a more widely available energy source, sunlight, although initially the photosynthesizing mats still depended on the diffusion of chemicals emitted by hydrothermal vents. The final and most significant stage of this liberation was the development of oxygen-producing photosynthesis, since the main chemical inputs for this are carbon dioxide and water.

As a result, microbial mats began to produce the atmosphere we know today, in which free oxygen is a vital component. At around the same time they may also have been the birthplace of the more complex eukaryote type of cell, of which all multicellular organisms are composed. Microbial mats were abundant on the shallow seabed until the Cambrian substrate revolution, when animals living in shallow seas increased their burrowing capabilities and thus broke up the surfaces of mats and let oxygenated water into the deeper layers, poisoning the oxygen-intolerant micro-organisms that lived there. Although this revolution drove mats off soft floors of shallow seas, they still flourish in many environments where burrowing is limited or impossible, including rocky seabeds and shores, hyper-saline and brackish lagoons, and are found on the floors of the deep oceans.

Because of microbial mats' ability to use almost anything as "food", there is considerable interest in industrial uses of mats, especially for water treatment and for cleaning up pollution.

Description

Stromatolites are formed by some microbial mats as the microbes slowly move upwards to avoid being smothered by sediment.

Microbial mats have also been referred to as "algal mats" and "bacterial mats" in older scientific literature. They are a type of biofilm that is large enough to see with the naked eye and robust enough to survive moderate physical stresses. These colonies of bacteria form on surfaces at many types of interface, for example between water and the sediment or rock at the bottom, between air and rock or sediment, between soil and bed-rock, etc. Such interfaces form vertical chemical gradients, i.e. vertical variations in chemical composition, which make different levels suitable for different types of bacteria and thus divide microbial mats into layers, which may be sharply defined or may merge more gradually into each other.[2] A variety of microbes are able to transcend the limits of diffusion by using "nanowires" to shuttle electrons from their metabolic reactions up to two centimetres deep in the sediment – for example, electrons can be transferred from reactions involving hydrogen sulfide deeper within the sediment to oxygen in the water, which acts as an electron acceptor.[3]

The best-known types of microbial mat may be flat laminated mats, which form on approximately horizontal surfaces, and stromatolites, stubby pillars built as the microbes slowly move upwards to avoid being smothered by sediment deposited on them by water. However, there are also spherical mats, some on the outside of pellets of rock or other firm material and others inside spheres of sediment.[2]

Structure

A microbial mat consists of several layers, each of which is dominated by specific types of micro-organism, mainly bacteria. Although the composition of individual mats varies depending on the environment, as a general rule the by-products of each group of micro-organisms serve as "food" for other groups. In effect each mat forms its own food chain, with one or a few groups at the top of the food chain as their by-products are not consumed by other groups. Different types of micro-organism dominate different layers based on their comparative advantage for living in that layer. In other words, they live in positions where they can out-perform other groups rather than where they would absolutely be most comfortable — ecological relationships between different groups are a combination of competition and co-operation. Since the metabolic capabilities of bacteria (what they can "eat" and what conditions they can tolerate) generally depend on their phylogeny (i.e. the most closely related groups have the most similar metabolisms), the different layers of a mat are divided both by their different metabolic contributions to the community and by their phylogenetic relationships.

In a wet environment where sunlight is the main source of energy, the uppermost layers are generally dominated by aerobic photosynthesizing cyanobacteria (blue-green bacteria whose color is caused by their having chlorophyll), while the lowest layers are generally dominated by anaerobic sulfate-reducing bacteria.[4] Sometimes there are intermediate (oxygenated only in the daytime) layers inhabited by facultative anaerobic bacteria. For example, in hypersaline ponds near Guerrero Negro (Mexico) various kind of mats were explored. There are some mats with a middle purple layer inhabited by photosynthesizing purple bacteria.[5] Some other mats have a white layer inhabited by chemotrophic sulfide-oxidizing bacteria and beneath them an olive layer inhabited by photosynthesizing green sulfur bacteria and heterotrophic bacteria.[6] However, this layer structure is not changeless during a day: some species of cyanobacteria migrate to deeper layers at morning, and go back at evening, to avoid intensive solar light and UV radiation at mid-day.[6][7]

Microbial mats are generally held together and bound to their substrates by slimy extracellular polymeric substances which they secrete. In many cases some of the bacteria form filaments (threads), which tangle and thus increase the colonies' structural strength, especially if the filaments have sheaths (tough outer coverings).[2]

This combination of slime and tangled threads attracts other micro-organisms which become part of the mat community, for example protozoa, some of which feed on the mat-forming bacteria, and diatoms, which often seal the surfaces of submerged microbial mats with thin, parchment-like coverings.[2]

Marine mats may grow to a few centimeters in thickness, of which only the top few millimeters are oxygenated.[8]

Types of environment colonized

Underwater microbial mats have been described as layers that live by exploiting and to some extent modifying local chemical gradients, i.e. variations in the chemical composition. Thinner, less complex biofilms live in many sub-aerial environments, for example on rocks, on mineral particles such as sand, and within soil. They have to survive for long periods without liquid water, often in a dormant state. Microbial mats that live in tidal zones, such as those found in the Sippewissett salt marsh, often contain a large proportion of similar micro-organisms that can survive for several hours without water.[2]

Microbial mats and less complex types of biofilm are found at temperature ranges from –40 °C to +120 °C, because variations in pressure affect the temperatures at which water remains liquid.[2]

They even appear as endosymbionts in some animals, for example in the hindguts of some echinoids.[9]

Ecological and geological importance

Wrinkled Kinneyia-type sedimentary structures formed beneath cohesive microbial mats in peritidal zones.[10] The image shows the location, in the Burgsvik beds of Sweden, where the texture was first identified as evidence of a microbial mat.[11]
Kinneyia-like structure in the Grimsby Foramtion (Silurian) exposed in Niagara Gorge, New York.

Microbial mats use all of the types of metabolism and feeding strategy that have evolved on Earth—anoxygenic and oxygenic photosynthesis; anaerobic and aerobic chemotrophy (using chemicals rather than sunshine as a source of energy); organic and inorganic respiration and fermentation (i..e converting food into energy with and without using oxygen in the process); autotrophy (producing food from inorganic compounds) and heterotrophy (producing food only from organic compounds, by some combination of predation and detritivory).[2]

Most sedimentary rocks and ore deposits have grown by a reef-like build-up rather than by "falling" out of the water, and this build-up has been at least influenced and perhaps sometimes caused by the actions of microbes. Stromatolites, bioherms (domes or columns similar internally to stromatolites) and biostromes (distinct sheets of sediment) are among such microbe-influenced build-ups.[2] Other types of microbial mat have created wrinkled "elephant skin" textures in marine sediments, although it was many years before these textures were recognized as trace fossils of mats.[11] Microbial mats have increased the concentration of metal in many ore deposits, and without this it would not be feasible to mine them—examples include iron (both sulfide and oxide ores), uranium, copper, silver and gold deposits.[2]

Role in the history of life

History of life
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A very brief history of life on Earth.
Axis scale is in millions of years ago.
Further information: Timeline of evolution

The earliest mats

Microbial mats are among the oldest clear signs of life, as microbially induced sedimentary structures (MISS) formed 3,480 million years ago have been found in western Australia.[2][12][13] At that early stage the mats' structure may already have been similar to that of modern mats that do not include photosynthesizing bacteria. It is even possible that non-photosynthesizing mats were present as early as 4,000 million years ago. If so, their energy source would have been hydrothermal vents (high-pressure hot springs around submerged volcanoes), and the evolutionary split between bacteria and archea may also have occurred around this time.[14]

The earliest mats were probably small, single-species biofilms of chemotrophs that relied on hydrothermal vents to supply both energy and chemical "food". Within a short time (by geological standards) the build-up of dead micro-organisms would have created an ecological niche for scavenging heterotrophs, possibly methane-emitting and sulfate-reducing organisms that would have formed new layers in the mats and enriched their supply of biologically useful chemicals.[14]

Photosynthesis

It is generally thought that photosynthesis, the biological generation of energy from light, evolved shortly after 3,000 million years ago.[14] However an isotope analysis suggests that oxygenic photosynthesis may have been widespread as early as 3,500 million years ago.[14] The eminent researcher into Earth's earliest life, William Schopf, argues that, if one did not know their age, one would classify some of the fossil organisms in Australian stromatolites from 3,500 million years ago as cyanobacteria, which are oxygen-producing photosynthesizers.[15] There are several different types of photosynthetic reaction, and analysis of bacterial DNA indicates that photosynthesis first arose in anoxygenic purple bacteria, while the oxygenic photosynthesis seen in cyanobacteria and much later in plants was the last to evolve.[16]

The earliest photosynthesis may have been powered by infra-red light, using modified versions of pigments whose original function was to detect infra-red heat emissions from hydrothermal vents. The development of photosynthetic energy generation enabled the micro-organisms first to colonize wider areas around vents and then to use sunlight as an energy source. The role of the hydrothermal vents was now limited to supplying reduced metals into the oceans as a whole rather than being the main supporters of life in specific locations.[16] Heterotrophic scavengers would have accompanied the photosynthesizers in their migration out of the "hydrothermal ghetto".[14]

The evolution of purple bacteria, which do not produce or use oxygen but can tolerate it, enabled mats to colonize areas that locally had relatively high concentrations of oxygen, which is toxic to organisms that are not adapted to it.[17] Microbial mats would have been separated into oxidized and reduced layers, and this specialization would have increased their productivity.[14] It may be possible to confirm this model by analyzing the isotope ratios of both carbon and sulfur in sediments laid down in shallow water.[14]

The last major stage in the evolution of microbial mats was the appearance of cyanobacteria, photsynthesizers which both produce and use oxygen. This gave undersea mats their typical modern structure: an oxygen-rich top layer of cyanobacteria; a layer of photsynthesizing purple bacteria that could tolerate oxygen; and oxygen-free, H2S-dominated lower layers of heterotrophic scavengers, mainly methane-emitting and sulfate-reducing organisms.[14]

It is estimated that the appearance of oxygenic photosynthesis increased biological productivity by a factor of between 100 and 1,000. All photosynthetic reactions require a reducing agent, but the significance of oxygenic photosynthesis is that it uses water as a reducing agent, and water is much more plentiful than the geologically produced reducing agents on which photosynthesis previously depended. The resulting increases in the populations of photosynthesizing bacteria in the top layers of microbial mats would have caused corresponding population increases among the chemotrophic and heterotrophic micro-organisms that inhabited the lower layers and which fed respectively on the by-products of the photosynthesizers and on the corpses and / or living bodies of the other mat organisms. These increases would have made microbial mats the planet's dominant ecosystems. From this point onwards life itself produced significantly more of the resources it needed than did geochemical processes.[18]

Oxygenic photosynthesis in microbial mats would also have increased the free oxygen content of the Earth's atmosphere, both directly by emitting oxygen and because the mats emitted molecular hydrogen (H2), some of which would have escaped from the Earth's atmosphere before it could re-combine with free oxygen to form more water. Microbial mats thus played a major role in the evolution of organisms which could first tolerate free oxygen and then use it as an energy source.[18] Oxygen is toxic to organisms that are not adapted to it, but greatly increases the metabolic efficiency of oxygen-adapted organisms[17] — for example anaerobic fermentation produces a net yield of two molecules of adenosine triphosphate, cells' internal "fuel", per molecule of glucose, while aerobic respiration produces a net yield of 36.[19] The oxygenation of the atmosphere was a prerequisite for the evolution of the more complex eukaryote type of cell, from which all multicellular organisms are built.[20]

Cyanobacteria have the most complete biochemical "toolkits" of all the mat-forming organisms: the photosynthesis mechanisms of both green bacteria and purple bacteria; oxygen production; and the Calvin cycle, which converts carbon dioxide and water into carbohydrates and sugars. It is likely that they acquired many of these sub-systems from existing mat organisms, by some combination of horizontal gene transfer and endosymbiosis followed by fusion. Whatever the causes, cyanobacteria are the most self-sufficient of the mat organisms and were well-adapted to strike out on their own both as floating mats and as the first of the phytoplankton, which forms the basis of most marine food chains.[14]

Origin of eukaryotes

The time at which eukaryotes first appeared is still uncertain: there is reasonable evidence that fossils dated between 1,600 million years ago and 2,100 million years ago represent eukaryotes,[21] but the presence of steranes in Australian shales may indicate that eukaryotes were present 2,700 million years ago.[22] There is still debate about the origins of eukaryotes, and many of the theories focus on the idea that a bacterium first became an endosymbiont of an anaerobic archean and then fused with it to become one organism. If such endosymbiosis was an important factor, microbial mats would have encouraged it. There are two possible variations of this scenario:

Life on land

Microbial mats from ~1,200 million years ago provide the first evidence of life in the terrestrial realm.[24]

The earliest multicellular "animals"

Before:
After:
Sessile organism
anchored in mat
Animal grazing
on mat
Animals embedded
in mat
Animals
burrowing
just under
mat
    =Microbial mat
Firm, layered, anoxic, sulphidic substrate
Animals moving on / in
surface of sea-floor
Loose,
oxygenated
upper substrate
with
burrowing
animals
Before and after the Cambrian substrate revolution

The Ediacara biota are the earliest widely accepted evidence of multicellular "animals". Most Ediacaran strata with the "elephant skin" texture characteristic of microbial mats contain fossils, and Ediacaran fossils are hardly ever found in beds that do not contain these microbial mats.[25] Adolf Seilacher categorized the "animals" as: "mat encrusters", which were permanently attached to the mat; "mat scratchers", which grazed the surface of the mat without destroying it; "mat stickers", suspension feeders that were partially embedded in the mat; and "undermat miners", which burrowed underneath the mat and fed on decomposing mat material.[26]

The Cambrian substrate revolution

In the Early Cambrian, however, organisms began to burrow vertically for protection or food, breaking down the microbial mats, and thus allowing water and oxygen to penetrate a considerable distance below the surface and kill the oxygen-intolerant micro-organisms in the lower layers. As a result of this Cambrian substrate revolution, marine microbial mats are confined to environments in which burrowing is non-existent or negligible:[27] very harsh environments, such as hyper-saline lagoons or brackish estuaries, which are uninhabitable for the burrowing organisms that broke up the mats;[28] rocky "floors" which the burrowers cannot penetrate;[27] the depths of the oceans, where burrowing activity today is at a similar level to that in the shallow coastal seas before the revolution.[27]

Current status

Although the Cambrian substrate revolution opened up new niches for animals, it was not catastrophic for microbial mats, but it did greatly reduce their extent.

How microbial mats help paleontologists

Most fossils preserve only the hard parts of organisms, e.g. shells. The rare cases where soft-bodied fossils are preserved (the remains of soft-bodied organisms and also of the soft parts of organisms for which only hard parts such as shells are usually found) are extremely valuable because they provide information about organisms that are hardly ever fossilized and much more information than is usually available about those for which only the hard parts are usually preserved.[29] Microbial mats help to preserve soft-bodied fossils by:

Industrial uses

The ability of microbial mat communities to use a vast range of "foods" has recently led to interest in industrial uses. There have been trials of microbial mats for purifying water, both for human use and in fish farming,[31][32] and studies of their potential for cleaning up oil spills.[33] As a result of the growing commercial potential, there have been applications for and grants of patents relating to the growing, installation and use of microbial mats, mainly for cleaning up pollutants and waste products.[34]

See also

Notes

  1. ↑ Schieber, J.; Bose, P, Eriksson, P. G.; Banerjee, S.; Sarkar, S.; Altermann, W.; Catuneanu, O. (2007). Atlas of Microbial Mat Features Preserved within the Siliciclastic Rock Record. Elsevier. ISBN 0-444-52859-8. Retrieved 2008-07-01.
  2. 1 2 3 4 5 6 7 8 9 10 Krumbein, W.E., Brehm, U., Gerdes, G., Gorbushina, A.A., Levit, G. and Palinska, K.A. (2003). "Biofilm, Biodictyon, Biomat Microbialites, Oolites, Stromatolites, Geophysiology, Global Mechanism, Parahistology". In Krumbein, W.E.; Paterson, D.M.; Zavarzin, G.A. Fossil and Recent Biofilms: A Natural History of Life on Earth (PDF). Kluwer Academic. pp. 1–28. ISBN 1-4020-1597-6. Archived from the original (PDF) on January 6, 2007. Retrieved 2008-07-09.
  3. ↑ Nielsen, L.; Risgaard-Petersen, N.; Fossing, H.; Christensen, P.; Sayama, M. (2010). "Electric currents couple spatially separated biogeochemical processes in marine sediment". Nature. 463 (7284): 1071–1074. Bibcode:2010Natur.463.1071N. doi:10.1038/nature08790. PMID 20182510. Lay summary – Nature News: Bacteria buzzing in the seabed.
  4. ↑ Risatti, J,B.; Capman, W.C.; Stahl, D.A. (October 11, 1994). "Community structure of a microbial mat: the phylogenetic dimension" (PDF). Proceedings of the National Academy of Sciences. 91 (21): 10173–7. Bibcode:1994PNAS...9110173R. doi:10.1073/pnas.91.21.10173. PMC 44980Freely accessible. PMID 7937858.
  5. ↑ Lucas J. Stal: Physiological ecology of cyanobacteria in microbial mats and other communities, New Phytologist (1995), 131, 1–32
  6. 1 2 Garcia-Pichel F., Mechling M., Castenholz R.W., Diel Migrations of Microorganisms within a Benthic, Hypersaline Mat Community, Appl. and Env. Microbiology, May 1994, pp. 1500–1511
  7. ↑ Bebout B.M., Garcia-Pichel F., UV B-Induced Vertical Migrations of Cyanobacteria in a Microbial Mat, Appl. Environ. Microbiol., Dec 1995, 4215–4222, Vol 61, No. 12
  8. ↑ Che, L.M.; Andréfouët. S.; Bothorel, V.; Guezennec, M.; Rougeaux, H.; Guezennec, J.; Deslandes, E.; Trichet, J.; Matheron, R.; Le Campion, T.; Payri, C.; Caumette, P. (2001). "Physical, chemical, and microbiological characteristics of microbial mats (KOPARA) in the South Pacific atolls of French Polynesia". Canadian Journal of Microbiology. 47 (11): 994–1012. doi:10.1139/cjm-47-11-994. PMID 11766060. Retrieved 2008-07-18.
  9. ↑ Temara, A.; de Ridder, C.; Kuenen, J.G.; Robertson, L.A. (February 1993). "Sulfide-oxidizing bacteria in the burrowing echinoid, Echinocardium cordatum (Echinodermata)". Marine Biology. 115 (2): 179. doi:10.1007/BF00346333.
  10. ↑ Porada H.; Ghergut J.; Bouougri El H. (2008). "Kinneyia-Type Wrinkle Structures—Critical Review And Model Of Formation.". PALAIOS. 23 (2): 65–77. doi:10.2110/palo.2006.p06-095r.
  11. 1 2 Manten, A.A. (1966). "Some problematic shallow-marine structures". Marine Geol. 4 (3): 227–232. doi:10.1016/0025-3227(66)90023-5. Retrieved 2007-06-18.
  12. ↑ Borenstein, Seth (13 November 2013). "Oldest fossil found: Meet your microbial mom". AP News. Retrieved 15 November 2013.
  13. ↑ Noffke, Nora; Christian, Christian; Wacey, David; Hazen, Robert M. (8 November 2013). "Microbially Induced Sedimentary Structures Recording an Ancient Ecosystem in the ca. 3.48 Billion-Year-Old Dresser Formation, Pilbara, Western Australia". Astrobiology (journal). 13 (12): 1103–24. Bibcode:2013AsBio..13.1103N. doi:10.1089/ast.2013.1030. PMC 3870916Freely accessible. PMID 24205812. Retrieved 15 November 2013.
  14. 1 2 3 4 5 6 7 8 9 10 11 Nisbet, E.G. & Fowler, C.M.R. (December 7, 1999). "Archaean metabolic evolution of microbial mats". Proceedings of the Royal Society B. 266 (1436): 2375. doi:10.1098/rspb.1999.0934. PMC 1690475Freely accessible. – abstract with link to free full content (PDF)
  15. ↑ Schopf, J.W. (1992). "Geology and Paleobiology of the Archean Earth". In Schopf, J.W.; Klein, C. The Proterozoic Biosphere: A Multidisciplinary Study. Cambridge University Press. ISBN 0-521-36615-1. Retrieved 2008-07-17.
  16. 1 2 Blankenship, R.E. (1 January 2001). "Molecular evidence for the evolution of photosynthesis". Trends in Plant Science. 6 (1): 4–6. doi:10.1016/S1360-1385(00)01831-8. PMID 11164357.
  17. 1 2 Abele, D. (7 November 2002). "Toxic oxygen: The radical life-giver". Nature. 420 (27): 27. Bibcode:2002Natur.420...27A. doi:10.1038/420027a. PMID 12422197. Retrieved 2008-07-14.
  18. 1 2 Hoehler, T.M.; Bebout, B.M.; Des Marais, D.J. (19 July 2001). "The role of microbial mats in the production of reduced gases on the early Earth". Nature. 412 (6844): 324–7. doi:10.1038/35085554. PMID 11460161. Retrieved 2008-07-14.
  19. ↑ "Introduction to Aerobic Respiration". University of California, Davis. Archived from the original on September 8, 2008. Retrieved 2008-07-14.
  20. ↑ Hedges, S.B.; Blair, J.E; Venturi, M.L.; Shoe, J.L (28 January 2004). "A molecular timescale of eukaryote evolution and the rise of complex multicellular life". BMC Evolutionary Biology. 4: 2. doi:10.1186/1471-2148-4-2. PMC 341452Freely accessible. PMID 15005799. Retrieved 2008-07-14.
  21. ↑ Knoll, Andrew H.; Javaux, E.J; Hewitt, D.; Cohen, P. (2006). "Eukaryotic organisms in Proterozoic oceans". Philosophical Transactions of the Royal Society B. 361 (1470): 1023–38. doi:10.1098/rstb.2006.1843. PMC 1578724Freely accessible. PMID 16754612.
  22. ↑ Brocks, J.J.; Logan, G.A.; Buick, R.; Summons, R.E. (13 August 1999). "Archean Molecular Fossils and the Early Rise of Eukaryotes". Science. 285 (5430): 1033–6. doi:10.1126/science.285.5430.1033. PMID 10446042. Retrieved 2008-07-16.
  23. ↑ Martin W. & Müller, M. (March 1998). "The hydrogen hypothesis for the first eukaryote". Nature. 392 (6671): 37–41. Bibcode:1998Natur.392...37M. doi:10.1038/32096. PMID 9510246. Retrieved 2008-07-16.
  24. ↑ Prave, A. R. (2002). "Life on land in the Proterozoic: Evidence from the Torridonian rocks of northwest Scotland". Geology. 30 (9): 811–812. doi:10.1130/0091-7613(2002)030<0811:LOLITP>2.0.CO;2. ISSN 0091-7613.
  25. ↑ Runnegar, B.N.; Fedonkin, M.A. (1992). "Proterozoic metazoan body fossils". In Schopf, W.J.; Klein, C. The Proterozoic biosphere. The Proterozoic Biosphere, a Multidisciplinary Study: Cambridge University Press, New York. Cambridge University Press. pp. 369–388. ISBN 978-0-521-36615-1.
  26. ↑ Seilacher, A. (1999). "Biomat-related lifestyles in the Precambrian". PALAIOS. PALAIOS, Vol. 14, No. 1. 14 (1): 86–93. doi:10.2307/3515363. JSTOR 3515363. Retrieved 2008-07-17.
  27. 1 2 3 Bottjer, D.J., Hagadorn, J.W., and Dornbos, S.Q. (2000). "The Cambrian substrate revolution" (PDF). 10: 1–9. Retrieved 2008-06-28.
  28. ↑ Seilacher, Adolf; Luis A. Buatoisb; M. Gabriela Mángano (2005-10-07). "Trace fossils in the Ediacaran–Cambrian transition: Behavioral diversification, ecological turnover and environmental shift". Palaeogeography Palaeoclimatology Palaeoecology. 227 (4): 323–56. doi:10.1016/j.palaeo.2005.06.003.
  29. 1 2 3 4 5 Briggs, D.E.G. (2003). "The role of biofilms in the fossilization of non-biomineralized tissues". In Krumbein, W.E.; Paterson, D.M.; Zavarzin, G.A. Fossil and Recent Biofilms: A Natural History of Life on Earth. Kluwer Academic. pp. 281–290. ISBN 1-4020-1597-6. Retrieved 2008-07-09.
  30. ↑ Seilacher, A. (1994). "How valid is Cruziana Stratigraphy?" (PDF). International Journal of Earth Sciences. 83 (4): 752–8. Bibcode:1994GeoRu..83..752S. doi:10.1007/BF00251073. Retrieved 2007-09-09.
  31. ↑ Potts, D.A.; Patenaude, E.L.; Görres, J.H.; Amador, J.A. "Wastewater Renovation and Hydraulic Performance of a Low Profile Leaching System" (PDF). GeoMatrix, Inc. Retrieved 2008-07-17.
  32. ↑ Bender, J (August 2004). "A waste effluent treatment system based on microbial mats for black sea bass Centropristis striata recycled-water mariculture" (PDF). Aquacultural Engineering. 31 (1–2): 73–82. doi:10.1016/j.aquaeng.2004.02.001. Retrieved 2008-07-17.
  33. ↑ "Role of microbial mats in bioremediation of hydrocarbon polluted coastal zones". ISTworld. Retrieved 2008-07-17.
  34. ↑ "Compositions and methods of use of constructed microbial mats – United States Patent 6033559". Retrieved 2008-07-17.; "Silage-microbial mat system and method – United States Patent 5522985". Retrieved 2008-07-17.; "GeoMat". GeoMatrix, Inc. Retrieved 2008-07-17. cites U.S. Patents 7351005 and 7374670

References

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