List of Dutch Top 40 number-one singles of 2000
These hits topped the Dutch Top 40 in 2000 (see 2000 in music).
| Issue Date | Artist | Song |
| 1 January | Marco Borsato  | Binnen |
| 8 January | Marco Borsato | Binnen |
| 15 January | Marco Borsato | Binnen |
| 22 January | Scoop | Drop It |
| 29 January | Scoop | Drop It |
| 5 February | Scoop | Drop It |
| 12 February | Abel | Onderweg |
| 19 February | Abel | Onderweg |
| 26 February | Abel | Onderweg |
| 4 March | Abel | Onderweg |
| 11 March | Abel | Onderweg |
| 18 March | Abel | Onderweg |
| 25 March | Bomfunk MC's | Freestyler |
| 1 April | Bomfunk MC's | Freestyler |
| 8 April | Bomfunk MC's | Freestyler |
| 15 April | Bomfunk MC's | Freestyler |
| 22 April | Bomfunk MC's | Freestyler |
| 29 April | Melanie C feat. Lisa "Left Eye" Lopes | Never Be the Same Again |
| 6 May | Melanie C feat. Lisa "Left Eye" Lopes | Never Be the Same Again |
| 13 May | Britney Spears | Oops!... I Did It Again |
| 20 May | Britney Spears | Oops!... I Did It Again |
| 27 May | Bon Jovi | It's My Life |
| 3 June | Bon Jovi | It's My Life |
| 10 June | Jop | Jij bent de zon |
| 17 June | Jop | Jij bent de zon |
| 24 June | Jop | Jij bent de zon |
| 1 July | Jop | Jij bent de zon |
| 8 July | Jody Bernal | Que si, que no |
| 15 July | Jody Bernal | Que si, que no |
| 22 July | Jody Bernal | Que si, que no |
| 29 July | Jody Bernal | Que si, que no |
| 5 August | Jody Bernal | Que si, que no |
| 12 August | Krezip | I Would Stay |
| 19 August | Krezip | I Would Stay |
| 26 August | Krezip | I Would Stay |
| 2 September | Melanie C | I Turn to You |
| 9 September | Melanie C | I Turn to You |
| 16 September | Melanie C | I Turn to You |
| 23 September | Melanie C | I Turn to You |
| 30 September | Enrique Iglesias & Whitney Houston | Could I Have This Kiss Forever |
| 7 October | Jody Bernal | Que si, que no |
| 14 October | Jody Bernal | Que si, que no |
| 21 October | U2 | Beautiful Day |
| 28 October | U2 | Beautiful Day |
| 4 November | Twarres | Wêr bisto |
| 11 November | Twarres | Wêr bisto |
| 18 November | Twarres | Wêr bisto |
| 25 November | Twarres | Wêr bisto |
| 2 December | Twarres | Wêr bisto |
| 9 December | Twarres | Wêr bisto |
| 16 December | LeAnn Rimes | Can't Fight the Moonlight |
| 23 December | LeAnn Rimes | Can't Fight the Moonlight |
| 30 December | LeAnn Rimes | Can't Fight the Moonlight |
See also
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