Hyaluronate lyase
| hyaluronate lyase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 4.2.2.1 | ||||||||
| CAS number | 37259-53-3 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / EGO | ||||||||
| |||||||||
In enzymology, a hyaluronate lyase (EC 4.2.2.1) is an enzyme that catalyzes the chemical reaction
- Cleaves hyaluronate chains at a beta-D-GalNAc-(1->4)-beta-D-GlcA bond, ultimately breaking the polysaccharide down to 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine
This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on polysaccharides. The systematic name of this enzyme class is hyaluronate lyase. Other names in common use include hyaluronidase [but cf. internal_xref(ec_num(3,2,1,35)), (hyalurononglucosaminidase) and internal_xref(ec_num(3,2,1,36)), (hyaluronoglucuronidase)], glucuronoglycosaminoglycan lyase, spreading factor, and mucinase.
Structural studies
As of late 2007, 27 structures have been solved for this class of enzymes, with PDB accession codes 1C82, 1EGU, 1F1S, 1F9G, 1I8Q, 1LOH, 1LXK, 1LXM, 1N7N, 1N7O, 1N7P, 1N7Q, 1N7R, 1OJM, 1OJN, 1OJO, 1OJP, 1W3Y, 2BRP, 2BRV, 2BRW, 2C3F, 2DP5, 2PK1, 2YVV, 2YW0, and 2YX2.
References
- Linker A, Hoffman P, Meyer K, Sampson P, Korn ED (1960). "The formation of unsaturated disacharides from mucopoly-saccharides and their cleavage to alpha-keto acid by bacterial enzymes". J. Biol. Chem. 235: 3061–5. PMID 13762462.
- MEYER K, RAPPORT MM (1952). "Hyaluronidases". Adv. Enzymol. Relat. Subj. Biochem. 13: 199–236. PMID 14943668.
- Moran F, Nasuno S, Starr MP (1968). "Extracellular and intracellular polygllacturonic acid trans-eliminases of Erwinia carotovora". Arch. Biochem. Biophys. 123 (2): 298–306. doi:10.1016/0003-9861(68)90138-0. PMID 5642600.