HaCaT

HaCaT is a spontaneously transformed aneuploid immortal keratinocyte cell line from adult human skin,[1] widely used in scientific research.[2] HaCaT cells are utilized for their high capacity to differentiate and proliferate in vitro.[3] Their use in research allows for the characterization of human keratinocyte using a model that is reproducible and addresses issues such as short culture lifespan and variations between cell lines that would otherwise be encountered. These cells have allowed the characterization of several processes, such as their utilization as a model system for Vitamin D3 metabolism in the skin.[4]

References

  1. ↑ Boukamp, Petra; Petrussevska, Rule T.; Breitkreutz, Dirk; Hornung, Jiirgen; Markham, Alex; Fusenig, Norbert E. (March 1988). "Normal Keratinization in a Spontaneously Immortalized Aneuploid Human Keratinocyte Cell Line". The Journal of Cell Biology. 106 (3): 761–771. doi:10.1083/jcb.106.3.761. PMID 2450098.
  2. ↑ Schoop, Veronika M.; Mirancea, Nicolae; Fusenig, Norbert E. (1 March 1999). "Epidermal Organization and Differentiation of HaCaT Keratinocytes in Organotypic Coculture with Human Dermal Fibroblasts". Journal of Investigative Dermatology. 112 (3): 343–353. doi:10.1046/j.1523-1747.1999.00524.x.
  3. ↑ Schürer, N; Köhne, A; Schliep, V; Barlag, K; Goerz, G (Aug 1993). "Lipid composition and synthesis of HaCaT cells, an immortalized human keratinocyte line, in comparison with normal human adult keratinocytes.". Experimental Dermatology. 2 (4): 179–85. doi:10.1111/j.1600-0625.1993.tb00030.x. PMID 8162337.
  4. ↑ Lehmann, Bodo (1 January 1997). "HaCaT Cell Line as a Model System for Vitamin D3 Metabolism in Human Skin.". Journal of Investigative Dermatology. 108 (1): 78–82. doi:10.1111/1523-1747.ep12285640.

External links

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